TARGET ENRICHMENT

Modern genomics applications require researchers to apply next-gen sequencing not only to whole genomes, but also to selected subsets of genes and other chosen regions of interest. Purifying or otherwise enriching a subset of the genome for sequencing studies is often called genome partitioning. NCGR offers three partitioning methods: Agilent SureSelect, which uses RNA oligonucleotides to capture cognate sequences in a genomic sample by hybridization, RainDance RainStorm, which amplifies target sequences using an efficient microfluidics-based PCR system, and NimbleGen. NCGR offers a comprehensive service, from evaluating which partitioning method best suits a customer application, assistance with design of the enrichment, partitioning, and sequencing. Reports are provided showing the efficiency of partitioning and summarizing sequence variation found in the sequenced DNA.

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