Sequencing Center Overview

NCGR Advantage: At the National Center for Genome Resources (NCGR) we are dedicated to staying at the forefront of next generation sequencing technology and to developing the best bioinformatics applications available. We always strive to produce the best possible sequencing and analysis results for our collaborators and clients.

Sequencing Center & Contact Info: Please take a moment to look over our standard services and NCGR FAQ and feel free  to give us a call (505-995-4444) or email us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it anytime if you have any questions about our services or if you would like to initiate a project with us. We look forward to hearing from you!

Technology: The NCGR Sequencing Center operations utilize the Illumina HiSeq 2000, Illumina GAIIx, and Pacific BioSciences RS instruments.

Certified Service Provider (CS-Pro):NCGR is a CS-Pro for Illumina sequencing and for the Agilent SureSelect target selection technology.

Sequencing Services: The Sequencing Center offers sequencing and target (exome) enrichment services providing high-quality results:

• Illumina DNA, RNA, ChIP and small RNA sequencing, mate-pair sequencing, and Pacific Biosciences long read sequencing.

• Sequencing of customer-supplied Illumina libraries.

• Sequencing for de novo transcript assembly.

• Agilent SureSelect target enrichment for sequencing customized genomic regions.

Please contact This e-mail address is being protected from spambots. You need JavaScript enabled to view it for more information.

Analysis Services: The Sequencing Center is tightly integrated with the NCGR Informatics group to bring customers professional analyses:

• Genomic SNP and Small Indel Discovery:  Alignment of genomic read data to a genomic reference to predict single nucleotide polymorphisms (SNPs) and indels.

• RNA-Seq Variant and/or Expression Analysis (using Genomic or Transcriptomic references):  Alignment of RNA-Seq data to a reference, prediction of SNPs and small indels, and read-count based expression and differential expression reports.

• De novo Transcript Assembly and Annotation:  De Bruijn graph based transcript assembly and annotation of protein products.

Results: Our expert staff was acknowledged by being the first Illumina CS-Pro service provider in North America and the first in the world to break the 20 billion base pair barrier using a single Illumina flowcell (January 2009). NCGR was awarded the Bio-IT World Best Practices in Basic Research for our schizophrenia studies using NGS for biomedical advancement, and the Computerworld Laureate award for our NGS analysis software. Since then we have had premiere journal publications for our NGS and analysis work in research endeavors spanning diverse areas from animal and plant agricultural systems to human genetics.