Analysis Services

The NCGR Sequencing Center is tightly integrated with the NCGR Informatics group to bring customers professional analyses:

• Genomic SNP and Small Indel Discovery:  Alignment* of genomic read data to a genomic reference to predict single nucleotide polymorphisms (SNPs) and indels.
• RNA-Seq Variant and/or Expression Analysis (using Genomic or Transcriptomic references):  Alignment of RNA-Seq data to a reference, prediction of SNPs and small indels, and read-count based expression and differential expression reports.
• De novo Transcript Assembly and Annotation:  De Bruijn graph based transcript assembly and annotation of protein products.

Our experienced lab-operation staff manages a professional process:  Disciplined scoping and scheduling of your project, SOPs and QC checkpoints, and deft handling of your samples from library prep through sequencing.  We then apply our NCGR contaminant filtering pipeline, producing the cleanest data results available. Data is then delivered or additional informatics analysis commences.

*Alpheus is our award-winning analysis software for large genome, transcriptome and methylome sequencing studies based on next-generation sequence data. Alpheus is designed to be used by scientists who are not experts in bioinformatics, statistics, software engineering or IT and who want to identify differences between sets of samples.