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A High Throughput Method for Cellular Uptake and Biodistribution Analysis of Extracellular Vesicles

Extracellular vesicles (EVs) are membrane-bound nanoparticles that are secreted by most plant, animal, and bacterial cells. EVs play an important role in many physiological and pathological processes. EVs are promising drug delivery systems for genome editing tools, gene silencing therapies and anti-cancer drugs. However, methods to track EV uptake in cell culture and animals are inefficient. Therefore, there is a need to develop new high throughput methods for studying EV biodistribution and targeted delivery in a scalable and efficient way. We propose the use of Next Generation Sequencing and tangential flow filtration for measuring cell culture uptake and in vivo biodistribution of EVs. Successful implementation of our method will enable researchers to create comprehensive catalogues of EV delivery sites and trafficking behaviors that are not feasible with current technologies, and will greatly advance basic EV research.


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